International journal of biological and agricultural research

Therapeutic aptitude of fermented camel milk: case of antimicrobial activity of whey proteins

2025-01-12T09:35:14+01:00

The objective Whey proteins (WP) are highly functional elements containing bioactive peptides. The aim of this study is to evaluate the effect of spontaneous fermentation on the antimicrobial activity of WP in camel milk. Samples of the milk are analyzed in the raw state and after the fermentation. The pH and acidity are determined as well as the enumeration of lactic acid bacteria (LAB). The antimicrobial activity was carried out by the agar well diffusion assay, adapting the good method. The results shows that the fermentation process has shown that the pH value is decreasing and LAB show a significant increase during fermentation. The antimicrobial activity gave a very significant difference between fermented camel whey proteins (FCWP) and raw camel whey proteins (RCWP). Then the FCWP give zone inhibition (ZI) of 17, 16.5, 13.25 and 10.5 mm respectively for S. aureus, P. aeruginosa, K. pneumoniae and E. coli. In addition, the RCWP gives a ZI of 16, 9.5 mm respectively for S. aureus and P. aeruginosa, and 0 mm for K. pneumoniae and E. coli. These results indicate that the fermentation process induced by the development of LAB favors the proteolysis of WP, which increases the antimicrobial activity.

Ameliorative effect of Aristolochia longa and Aquilaria malaccensis on lead acetate induced nephrotoxicity in female rats

2025-01-12T08:57:05+01:00

Aquilaria malaccensis And Aristolochia longa are plants used extensively in traditional medicine for the treatment of cancer in several regions in Algeria. We investigate the ameliorative effect of Aristolochia longa and Aquilaria malaccensis on kidney toxicity induced by lead in female albino rats. Twenty-five (25) apparently healthy female Wistar rats were randomly divided into five groups of five rats in each: control, Pb, Pb + A. longa (Ar), Pb+ A. malaccensis (Aq), and Pb+Ar+Aq.lead (100 mg/kg b.w) as Pb(C2H3O2)2 added in their drinking water for 75 days. A. longa (rhizome powder at a dose 1% w/w of diet) and A. malaccensis (heartwood powder at a dose 1% w/w of diet) were added to the feed during the last 15 days of lead exposed in the animals. Obtained results revealed that lead treatment caused a significant increase in blood glucose level, serum urea, creatinine and uric acid level and in kidney of MDA level and CAT activity. In contrast, it led to a decrease in the kidney GST activity and GSH level in rats. Also, the results clearly showed that lead causes alterations of glomerular and tubular structure in comparison with controls. Our results showed that treatment with A. malaccensis and A. longa a partial correction of the previous parameters. The histological observations confirmed the nephroprotection results by the biochemical parameters. Results demonstrated beneficial effects of A. longa and A. malaccensis treatment again acetate lead-induced toxicity and kidney damage in rats.

Investigation of the Oxidative Stress markers in salivary, serum and erythrocyte of El-Oued (Algeria) Diabetic Patients

2025-01-11T10:29:55+01:00

The aim of this study was to evaluate the state of oxidative stress during diabetes in El-Oued (Algeria) patients. The experimentation is carried out on 72 voluntary individuals were divided into three groups. The first group of healthy individuals (controls), the second group diabetic type 1and the third group diabetic type 2, on which we measured some biochemical parameters on serum, erythrocytes and saliva. The Results showed a significant decrease in serum uric acid concentration in diabetics type 1 (10.78%) and diabetics type 2 (26.83%) and a significant decrease (p <0.05)in erythrocytes GSH in both types of diabetes compared to controls. On the other hand, there was a significant increase (p <0.05) in serum and salivary peroxidation lipidique (MDA) in type 1 and 2 diabetics compared to non-diabetics. In addition, a significant correlation (p

<0.05) between serum and salivary MDA in type 1 diabetics (R² = 0.750) and type 2 diabetics (R² = 0.768) was shown in this study. In conclusion the present study reveals that diabetes induces an imbalance of oxidant / antioxidant status at serum, erythrocyte and salivary level and that salivary MDA is a good indicator of the state of oxidative stress in diabetes.

Influence of culture media on the production of antibacterial compounds by Streptomyces sp. PAL114 isolated from palm grove soil in Ghardaïa

2025-01-11T09:57:50+01:00

A Saharan actinobacterium, PAL114, isolated from Ghardaïa (Algeria), showed an important antibacterial activity against various pathogenic bacteria tested on different culture media. The results showed that the highest antibacterial activity was obtained after 7 days of growth. The Bennett medium was found to be the best medium for optimal growth and antibiotic production. The secondary metabolites were detected by reverse-phase HPLC using a C18 column and the fractions corresponding to peaks were collected, concentrated then tested against Listeria monocytogenes ATCC 13932 to detect the active fractions.

Floristic diversity of Saharan wetlands in the region of Oued Righ, northeastern Sahara of Algeria

2025-01-11T08:54:00+01:00

The main objective of this study was to investigate the floristic composition and the vegetation structure of different Saharan wetlands in the region of Oued Righ. Six (6) Saharan wetlands were examined; the Lake of Merjaja, Lake of Megarine, Lake of Sidi Slimane, lake of Ayata, Lake Ain Zerga, and Oued Khrouf. A total of 25 stands were investigated using the quadrat method, with five stands for each wetland. The different vegetation parameters, such as cover, frequency and density were calculated. A total of 24 plant species belonging to 20 genera and 13 families were identified. Chenopodiaceae was the predominant family with 6 species. The majority of the species were of Saharo-Arabian distribution. Chemaephytes had the highest contribution to the life forms spectra. The potential role of the halophyte species is discussed. These results might contribute to a better understanding of the functions, requirements, and sensitivities of these ecosystems.