Allelopathic Influence of Rosmarinus officinalis Aqueous Extracts on Barley Plants


  • Muoftah A M Bataw -


Rosmarinus officinalis; allelopathic interactions; Aqueous Extracts Barley Plants.


This is an investigation of Allelopathic Influence of Rosmarinus officinalis Aqueous Extracts on Barley Plants. All the samples are prepared from Libyan varieties of these two species. Rosmarinus officinalis specimens were gathered, air-dried, and ground, yielding a 20% aqueous extract. Dilutions (16%, 12%, 8%, 4%) and a control (0% distilled water) were prepared. Dilution followed the equation C2×V2=C1×V1. For example, to achieve 16%, 12 ml of the extract mixed with 3 ml of distilled water. Petri dishes, cleaned meticulously, held five barley seeds each, receiving 5 ml of extract or distilled water. After a week in the lab with two irrigations, root and stem lengths were measured. Statistical analyses, including one-way ANOVA and multiple comparisons, were conducted using SPSS 28 to interpret the extract's impact. The results of the experiment revealed a significant difference in Barley root measurements among the experimental conditions, as evidenced by a significant F-statistic (F(4, 10) = 15.58, p < .001). The Between Groups comparison, with a sum of squares of 38.85 and 4 degrees of freedom, resulted in a mean square of 9.71. Results also revealed a significant difference in barley stem measurement among the experimental conditions, as indicated by a highly significant F-statistic (F(4, 10) = 45.777, p < .001). These differences indicate that the presence of Rosmarinus officinalis Weed extract, under various concentrations and conditions, distinctly influenced (negatively) the growth parameters of barley plants.


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How to Cite

Bataw, M. A. M. (2024). Allelopathic Influence of Rosmarinus officinalis Aqueous Extracts on Barley Plants. Algerian Journal of Biosciences, 5(01), 28–34. Retrieved from