Biochemical and histoarchitectural changes in the liver, kidney and heart after oral administration of Viscum album leaf extract in oestradiol valerate-treated female rats

Authors

  • Dr Falilat Adeola Oseni Yusuf Maitama Sule University, Kano
  • Professor Musa Toyin Yakubu
  • Professor Elizabeth Abidemi Balogun
  • Amadu Salau Federal University of Health Sciences, Ila-Orangun, Nigeria

Keywords:

Viscum album leaves, polycystic ovarian syndrome, metformin, oestradiol valerate, Loranthaceae

Abstract

Effect of Viscum album leaf aqueous extract (VALEA) on biochemical parameters of oestradiol valerate (OV)-treated female rats was assessed. Group A rats received 0.5 ml of distilled water (control), the OV-treated rats (Groups B – F) were respectively administered 0.5 ml of distilled water, metformin (2.4 mg/kg bdwt), 50, 100 and 200 mg/kg bdwt of VALEA for 30 days. OV treatment significantly (p<0.05) decreased liver and serum ALP, liver GGT and serum hexokinase. Also significantly reduced were levels of total bilirubin, urea, K+ and HDL. Activities of kidney ALP, liver and serum ALT, serum AST, serum GGT as well as unconjugated bilirubin, blood glucose, Na+, HCO3-, FFAs, triacylglycerols and VLDLs were elevated by OV treatment, which also induced liver fat and myocarditis. Significantly (p>0.05) unaffected by the OV treatment were liver AST, serum total proteins, albumin, globulin, conjugated bilirubin, creatinine, uric acid, Cl-, TC, atherogenic index and the kidney histoarchitecture. Administration of VALEA reversed the OV-induced biochemical alterations and the liver and cardiac histoarchitectural integrity in a manner that compared well with metformin in some parameters and doses. The results showed that VALEA has the potential to ameliorate OV-induced biochemical and histoarchitectural alterations in female rats.

References

Zhang J, Bao Y, Zhou X, Zheng L. Polycystic ovarian syndrome and mitochondrial dysfunction. Reproductive Biol Endocrinol 2019;17:67.

Mane AS. Correlation between serum sodium and potassium levels and risk of developing hypertension. Int J Contemp Med Res 2019;5(11):k5-k9.

Michael OA, Bimbola FM, Rotimi O. The relationship between measures of obesity and atherogenic lipids among Nigerians with hypertension. Malawi Med J 2019;(3):193-197.

Katuzna M, Cztapka-matyasik M, Kompf P, Janicki A, Ziemnicka K. Lipid ratios and obesity indices are effective predictors of metabolic syndrome in women with polycystic ovary syndrome. Ther Adv Endocrinol Metabol 2022;13:1-13.

Abasian Z, Rostamzadeh A, Mohammadi, M, Hosseini M, Rafieian-kopaei M. Review on role of medicinal plants in polycystic ovarian syndrome: pathophysiology, neuroendocrine signaling, therapeutic status and future prospects. Middle East Fertil Soc J 2018;23:255-262.

Azziz R. Polycystic ovarian syndrome. Obstet Gynecol 2018;132 (2):321-336.

De Leo V. Musacchio MC, Cappelli V, Massaro MG, Morgante G, Petraglia F. Genetic, hormonal and metabolic aspects of PCOS: An update. Reprod Biol Endo Rev 2016;14(1):38.

Kabiru M, Usman M, Manga SB, Opaluwa SA, Nura A. Phytochemical screening and antibacterial activity of the crude extract and fractions of Tapinanthus globiferus leaves on the bacterial isolates of wound. World J Pharm Rep 2017;6(7):209-238.

Ibe FC, Ibe BO, Enyoh CE. Trace metal, FTIR and phytochemical analysis of Viscum album leaves harvested from Pentaclethra macrophylla. World News Nat Sci 2019;25:61-71.

Szurpnicka A, Kowalczuk A, Szterk A. Biological activity of mistletoe in vitro and in vivo studies and mechanism of action. Arc Pharmacol Res 2020;43:593-629.

Brahma NS, Chaitrali S, Danijel G, Dalip KU, Srini VK. Viscum album: a potent phytotherapeutic agent with multifarious phytochemicals, pharmacological properties and clinical evidence. RSC Adv Royal Soc Chem 2016;6(28):23837-23892.

Chukwu CN, Onyedikachi UB, Ejiofor E. Evaluation of chemical constituents, antioxidant and anti-inflamatory properties of n-hexane extract of Viscum album (mistletoe) leaves. Chiang Mai Univ J Nat Sci 2022; 21(1):e2022010.

Turkkan A, Savas HB, Yavuz B, Yigit A, Kale B. The proprophylactic effect of Viscum album in streptozotocin-induced diabetic rats. Northern Clin Istanbul 2016;3(2):83-89.

Pechter U, Kalev I Ots-Rosenberg M. Renoprotective and blood pressure lowering impact of Equisetum arvense and Viscum album therapy in experimental model chronic kidney diseases. World J Cardio Dis 2018;8:545-556.

Paredes FM, Barrera AF, Alquicira EF, Ramos RR, Santos JS. Antihyperglycemic, antioxidant and antiflammatory effects of aqueous extract of mistletoe (Cladocora loniceroides) in STZ-induced diabetic mice. Afr J Complem Alt Med 2019;16(1): 1-12.

Park JH, Kim Y, Kim JK, Park HY Song BS. Viscothionin purified from mistletoe (Viscum album var. coloratum Ohwi) induces insulin secretion from pancreatic cells. J Ethnopharmacol 2019;234:172-179.

Lawal ET, Ekpruke CD, Abu E. Effects of mistletoe on the lipid profile and aorta of triton WR-1339 treated wistar rats. Annals Biomed Sci 2017;16(2): 271-289.

Oseni FA, Salau AK, Afolabi-Balogun NB, Balogun EA, Yakubu MT. Effects of aqueous extract of Viscum album (Linn.) leaves on some biochemical parameters and histological changes in oestradiol valerate-induced polycystic ovarian syndrome in rats. Int J Biol Chem Sci 2019;13(6): 2521-2532

Steinborn C, Klemd AM, Sanchez-Campillo AS, Rieger S, Schefen M, Sauer B, Garcia-Kaufer M, Ucker A, Kienle GS, Huber R, Grundemann C. Viscum album neutralizes tumor-induced immunosuppression in a human in vitro cell model. PLoSONE 2017;12:e0181553. https://doi. org/10.1371/journal.pone.0181553.

Esin SC, Hassan T, Ozgur IC, Niger Y, Ibrahim HC. Methotrexate-induced nephrotoxicity in rats; protective effect of mistletoe (Viscum album L.) extract. Complem Med Res 2017;24:364-370.

Roseline E, Biosa N, Tamuno-Emine DG. Therapeutic effect of Viscum album against CCL4-induced hepatotoxicity in male albino rats. World J Pharm Med Res 2018;4(7): 14-20.

Kortam M, Mohammad H, Mobarak M, Bazazo A. The effects of estradiol valerate with and without oral sildenafil on endometrial thickness and pregnancy rates in infertile women. A R.C.T. Evidence Based Women’s Health J 2018;8(4):306-310.

Onyszkiewicz M, Gawrys-Kopczynska M, Salagaj M, Aleksandrowicz M, Sawicka A, Koźniewska E et al. Valeric acid lowers arterial blood pressure in rats. European J Pharmacol 2020;877:173086. https://doi.org/10.1016/j.ejphar.2020.173086.

Yakubu MT, Akanji MA, Oladiji AT, Olatinwo AO, Adesokan AA, Yakubu MO et al. Effect of Cnidoscolous aconitifolius (Miller) I.M. Johnston leaf extract on reproductive hormones of female rats. Iranian J Repro Med 2008;6(3):149-155.

Gornall AC, Bardawill CJ, David MM. Determination of serum protein by means of biuret reaction. J Biol Chem 1949;177:751-756.

Doumas BT, Watson WA, Biggs HG. Albumin Standards and the Measurement of serum albumin with bromcresol green. Clinica Chimica Acta 1971;31(1):87-96.

Evelyn KA, Malloy HT. Micro determination of oxyhaemoglobin, methaemoglobin and sulphaemoglobin in a single sample of blood. J Biol Chem 1938;126:655-661.

Tietz NW. Clinical guide to laboratory tests. 4th ed. Philadelphia: WB Saunders Company; 1995.

Veniamin MP, Varkirtzi-Lemonias C. Chemical basis of the carbamidodi-acetyl micro-method for estimation of urea, citrulline and carbamyl derivatives. Clin Chem 1970;16(10):3-6.

Bartels MD, Bohmer PW. Biochemistry at a glance, 1972.

Wright PJ, Leathwood PD, Plummer DT. Enzymes in rat urine: alkaline phosphatase. Enzymologia 1972;42:317-327.

Reitman S, Frankel S. A colorimetric method for determination of serum glutamate-oxaloacetate and pyruvate transaminase. Am J Pathol 1957;28(1):56-63.

Szasz G. Kinetic photometric method for serum gamma-glutamyl transpeptidase. Clin Chem 1969;15(2):124-136.

Brandstrup N, Kirk JE, Bruni C. The hexokinase and phosphoglucoisomerase activities of aortic and pulmonary artery tissue in individuals of various ages. J Gerontol 1996;12:166-171.

Trinder P. Determination of glucose in blood using glucose oxidase with alternative oxygen acceptor. Annals Clin Biochem 1969;6:24-27.

Fredrickson DS, Levy RI, Lees RS. Fat transport in lipoproteins-an integrated approach to mechanisms and disorders. New Eng J Med 1967;276:148-156.

Friedewald WT, Levy RI, Friedrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972;18:499-502.

Junqueira LCU, Carneiro J, Kelley RO. Basic histology. 8th ed. Michigan: Appleton & Lange; 1995.

Ramasamy I. Persistent increase in serum alkaline phosphatase in a patient with monoclonal gammopathy of undefined significance. Case Rep Hematol 2020; Article ID 8406971, 5 pages. http://doi.org/10.1155/2020/8406971.

Wasserman DH. Insulin, muscle glucose uptake and hexokinase: revisiting the road not taken. Physiology (Bethesda) 2022;37(3):115-127. doi: 10.1152/physiol.00034.2021.

Adelakun AS, Ojewale AO, Jeje SO, Adedotun OA. Histomorphometric and biochemical activities of bioactive component of Cyperus esculentus tubers extract on letrozole-induced polycystic ovarian syndrome and cholesterol homeostasis in female Jenn Sprague-Dawley rats. Toxicol Res Appl 2022;6:1-18.

Memon N, Weinberger BI, Hegyi T, Aleksunes LM. Inherited disorders of bilirubin clearance. Pediatric Res 2016;79(3):378-385.

Willemars MMA, Nabben M, Verdonschot JAJ, Hoes MF. Evaluation of the interaction of sex hormones and cardiovascular function and health. Curr Heart Fail Rep 2022;19(4):200-212. doi: 10.1007/s11897-022-00555-0.

Ostermanni M, Kashani K, Forni GL. The two sides of creatinine: both as bad as each other. J Thor Dis 2016;(7):E628-E630.

Alahmadi AA, Alahmadi BA, Wahman LF, El-Shitany NA. Chamomile flower extract ameliorates biochemical and histological kidney dysfunction associated with polycystic ovarian syndrome. Saudi J Biol Sci 2021;28:6158-6166.

Staruschenko A. Beneficial effects of high potassium: contribution of renal basolateral K+ Channels. Hypertension 2018;(71):1015-1022.

Lou X, Cai WY, Wu XK. Prevalence, pattern and predictors for dyslipidemia of Chinese women with polycystic ovary syndrome. Front Cardiovasc Med 2021;8:790454. doi: 10:3389/fcvm.2021.790454.

Lim YC, Rajabalaya R, David SR. Parasitic mistletoes of the genera Scurrula and Viscum from bench to bedside. Molecules 2016;21(8):1048.

Strassheim D, Dempsey EC, Gerasimovskaya E, Stenmark K, Karoor V. Role of inflammatory cell subtypes in heart failure. J Immunol Res 2019;2164017. doi: 10.1155/2019/2164017.

Wang J, Han B. Dysregulated CD4+ T cells and microRNAs in myocarditis. Front Immunol 2020;11:539. https://doi.org/10.3389/fimmu.2020.00539.

Downloads

Published

2024-06-30

How to Cite

Oseni, F., Yakubu, M., Balogun, E., & Salau, A. (2024). Biochemical and histoarchitectural changes in the liver, kidney and heart after oral administration of Viscum album leaf extract in oestradiol valerate-treated female rats. Algerian Journal of Biosciences, 5(01), 001–012. Retrieved from https://journal.acse.science/index.php/ajb/article/view/161